The performance will raise money for LGBT charity Switchboard
Mark Gatiss and Ian Hallard will feature among a cast of West End performers in a one-off performance of Outings, as part of the Pride in London Festival this year.
The piece, which premiered at the Edinburgh Fringe in 2014, is a collation of 20 real life coming-out stories.
Tony Award-winner David Grindley will direct the show which also includes performances from Olivier Award-winner Nichola McAuliffe (Kiss Me, Kate) and comedians Zoe Lyons, Rob Deering, Andrew Doyle and Camille Ucan.
All proceeds from the evening will go to the LGBT+ helpline charity, Switchboard.
Outings will be held at the Lyric Theatre on Monday 26 June.