Directed by Joe Mantello, the new film will be released on 30 September on the streaming platform. It focusses on an uncomfortable birthday gathering amongst a group of gay friends on Manhattan's Upper East Side.
Based on the Tony Award-winning revival, the cast includes Jim Parsons, Matt Bomer, Zachary Quinto and Andrew Rannells.
You can watch the brand new trailer here:
The comedy, which first opened in 1968 and ran for over 1000 performances, was later adapted into a film in 1970, directed by William Friedkin. Mark Gatiss and Ian Hallard starred in a north London 2016 revival of the show, which transferred to the West End the following year.
Ryan Murphy has a series of stagey shows heading for Netflix, including The Prom big-screen adaptation and a new series based on A Chorus Line.