The 120-seater venue launches its Thursday comedy season on 11 October, with recent Edinburgh Fringe award-winner Marcel Lucont, who will co-headline with duo Frisky and Mannish. The Friday jazz season, curated by musician Dorian Ford, will open with blues singer Carol Grimes.
Associate artistic director James Albrecht said: "Our ambition is to make the St James Studio a major venue for London and the local area in and around Victoria. We aim to present the best in both established and up and coming comedy on Thursdays, to emerge as London's newest and most exciting Jazz venue on Fridays and bring you acclaimed cabaret on Saturdays."
Come Christmas-time, the studio will also host Clive Fracis' one-man version of A Christmas Carol from 10-16 December.
The theatre's long-anticipated grand opening will take place this September, with the main theatre's season beginning a few weeks before the studio season.